Vacanti Mouse

Vacanti mouse

The Vacanti mouse was a laboratory mouse (circa 1996) that had what looked like a human ear grown on its back. The "ear" was actually an ear-shaped cartilage structure grown by seeding cow cartilage cells into biodegradable ear-shaped mold and then implanted under the skin of the mouse, with an external ear-shaped splint to maintain the desired shape. Then the cartilage naturally grew by itself within the restricted shape and size. The splint was removed briefly to take the publicity pictures. The earmouse, as it became known, was created by Charles A. Vacanti in the Department of Anesthesiology at Massachusetts General Hospital and Harvard Medical School, Massachusetts General Hospital at Massachusetts Institute of Technology, Joseph P. Vacanti in the Department of Surgery at Children's Hospital. Vacanti[which?] later moved to the Department of Anesthesiology at the University of Massachusetts Medical School. The results were based on the works of many others who seeded cells onto scaffolds to regenerate organs. The first work with cartilage regeneration was published in 1991 in Plastic and Reconstructive Surgery. The mouse used is called a nude mouse, a commonly used strain of immunocompromised mouse, preventing a transplant rejection. The photo of the mouse was passed around the internet, mainly via email, sometimes with little to no text accompanying it leading many people to speculate whether the photo was real. In the late 1990s, the picture prompted a wave of protests against genetic engineering-although in this specific experiment no genetic manipulation was performed.

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In vivo imaging of metastatic mouse models

Transgenic mouse models can be imaged by various non-invasive techniques. Bioluminescence imagingBioluminescence imaging relies on the detection of light produced by the enzymatic oxidation of an exogenous substrate. The substrate luciferin, is oxidized to oxyluciferin in the presence of luciferase and emits light, which can be detected using an IVIS system such as a Xenogen machine. Dissociated mammary cells from MMTV-PyMT: IRES: Luc; MTB (Internal ribosome entry site: Luciferin) animals (which were not exposed to doxycycline) can be injected into the lateral tail veins of immunodeficient mice on a doxycycline-free diet. No bioluminescence signal will be observed in the lungs of recipient mice until they are given doxycycline food. Bioluminescence can then be detected in the chest within 2 weeks of the start of doxycycline exposure. Luciferase is injected just before taking the images. Fluorescent imagingIntravital microscopy with multi photon excitation is a technique to visualize genetically engineered cells directly in vivo. Multi step metastatic cascades can be visualized by labelling with unique fluorescent colour under fluorescence microscope. Radioisotopic imagingPositron emission tomography (PET), single photon emission computed tomography (SPECT) and computed tomography (CT) have been used to compare the efficiency of these in vivo imaging for detecting lesions at an early stage and to evaluate the response to chemotherapy. MRI ImagingMagnetic resonance imaging requires the use of nano-particles(liposomes) and an MRI contrast agent called gadolinium. The particles were then placed in vesicles via a polycarbonate membrane filter. The nano-particles are injected into the metastases evolved mice, and left there for twenty-four hours. These mice are then scanned, and in the imaging software there are accumulations of these particles in certain areas where cells have metastasized.

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Opened my laptop and now my mouse doesn't work?

TThose keys cant be fixed, you have to replace the keyboard, but as for the mouse i suspect that the ribbon cable has come off! Open the keyboard again and look for a loose ribbon cable and slot it back in

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Mighty Mouse (nickname)

As a nickname, Mighty Mouse may refer to: Eric Aiken (born 1980), American featherweight boxer and former IBF world champion Jimmy Alapag (born 1977), Philippine Basketball Association player Pam Barrett (1953-2008), Canadian politician Chuck Fenenbock (1917-1998), American National Football League and Canadian Football League player Shannon Gallant (born 1986), Australian rugby league player Demetrious Johnson (fighter) (born 1986), American mixed martial arts fighter and UFC Flyweight champion Kevin Keegan, English former footballer Tony Leswick (1923-2001), Canadian National Hockey League player Ian McLauchlan (born 1942), Scottish retired rugby union player Mark McMillian (born 1970), American retired National Football League player Kyla Ross (born 1996), American artistic gymnast Michael Russell (tennis) (born 1978), American tennis player Damon Stoudamire (born 1973), American retired National Basketball Association player Elaine Tanner (born 1951), Canadian retired swimmer Zhang Anda (born 1991), Chinese snooker player